Stages in an enzymecatalysed reaction

An enzyme-catalysed reaction can be considered to occur in three distinct steps, all of which are reversible:


Figure 2.5 Cis/trans isomerism.

  • Binding of the substrate (S) to the enzyme, to form the enzyme—substrate complex: Enz + S ^ Enz — S
  • Reaction of the enzyme-substrate complex to form the enzyme—product complex: Enz — S ^ Enz — P
  • Breakdown of the enzyme—product complex, with release of the product (P): Enz - P ^ Enz + P

Hence, overall, the process can be written as:

where Enz is the enzyme, S the substrate and P the product. The reaction occurs in three stages, all of which are reversible.

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