Histological examination

Histological analysis of small bowel biopsies shows a variable degree of villus atrophy without any inflammatory infiltrate. Highly characteristic for this disorder is the accumulation of periodic acid Schiff (PAS)-positive secretory granules within the apical cytoplasm of enterocytes.18-20 On

Pas Microvilli

the ultrastructural level, rare or absent microvilli on intestinal epithelial cells along with inclusions of microvilli in the cytoplasm of enterocytes are seen, which define this entity.21

Diagnosis may be easily performed from light microscopic examination of a duodenal or jejunal biopsy specimen. On hematoxylin-eosin staining, the mucosa appears flattened with hypoplastic villus atrophy. PAS staining reveals an abnormal brush-border pattern with positive-staining material within the apical cytoplasm of enterocytes (Figure 1.1). A valuable new tool for the light microscopic diagnosis of MVID was recently proposed.22,23 CD-10 is a membrane-associated neutral peptidase, shown to have a linear brush-border staining pattern in normal small intestine. In contrast to this surface staining in different controls (normal intestine, celiac disease, autoimmune enteropathy, allergy), all MVID cases revealed prominent intracytoplasmic CD-10 immunoreactivity in surface enterocytes22,23 (Figure 1.2). Similar results were obtained with PAS, polyclonal carcinoembryonic antigen and alkaline phosphatase, three stains known to show cytoplasmic staining of surface enterocytes in MVID.23 On electron microscopy, surface epithelial cells show absent or grossly abnormal microvilli, as well as numerous vesicular bodies of various sizes, and the characteristic microvillus inclusions (Figure 1.3). Crypt cells are morphologically almost normal, but do not contain increased

Microvillus Inclusion Disease

Figure 1.1 Microvillus inclusion disease: periodic acid-Schiff (PAS) staining. (a) Normal mucosa, normal PAS, brush-border staining; (b) abnormal accumulation of PAS, positive material in the apical cytoplasm of epithelial cells in microvillus inclusion disease.

Cd10 Microvillus InclusionNutritio Border
Figure 1.3 Microvillus inclusion disease. Electron microscopy of jejunal biopsy specimen. The brush border is almost absent. The cytoplasm contains a microvillus inclusion.
Microvillus Inclusion DiseaseMicrovillus Inclusion Disease
Figure 1.4 Microvillus inclusion disease. Electron microscopy of colonic biopsy specimen.

numbers of apical vesicles and vesicular bodies. Microvillus inclusions, as well as increased secretory granules, are also present in the large bowel, more easily accessible for biopsy, especially in early infancy (Figure 1.4).

Why Gluten Free

Why Gluten Free

What Is The Gluten Free Diet And What You Need To Know Before You Try It. You may have heard the term gluten free, and you may even have a general idea as to what it means to eat a gluten free diet. Most people believe this type of diet is a curse for those who simply cannot tolerate the protein known as gluten, as they will never be able to eat any food that contains wheat, rye, barley, malts, or triticale.

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