Problem Measurement of urine glucose

As discussed in section 4.2.1.4, glucose in plasma and urine can be determined in two ways, using an alkaline copper reagent or using glucose oxidase linked to a dyestuff. Both methods were used to determine urine glucose in six people:

A a person with hitherto undiagnosed diabetes mellitus (section 10.7); B a known diabetic patient, with poor glycaemic control, taking supplements of

500 mg of vitamin C per day; C a non-diabetic subject taking supplements of 500 mg of vitamin C per day; D a person with idiopathic pentosuria (excretion of five-carbon sugars in the urine); E a person with idiopathic pentosuria taking supplements of 500 mg of vitamin C per day;

F a non-diabetic subject taking no supplements.

Which subjects would be expected to give the following results?

  • positive using glucose oxidase, positive using alkaline copper reagent;
  • positive using glucose oxidase, negative using alkaline copper reagent;
  • negative using glucose oxidase, positive using alkaline copper reagent;
  • negative using glucose oxidase, negative using alkaline copper reagent.
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